By “artificial compound” is meant herein any molecule which happens to be neither The natural way made by the host issue (or by a subject of exactly the same species since the host species) nor by An additional organism, specifically an organism from One more species, from An additional gender, from another family, from One more course or from An additional kingdom.
A plasmid of sequence SEQ ID NO: 10, carrying a conditional origin of replication of sequence SEQ ID NO: 7, and coding for the nuclease and its guideline concentrating on the stx gene outlined earlier mentioned, was packaged into an engineered lambda-dependent capsid, comprising a chimeric 1A2 gpJ protein as well as a chimeric STF-V10[Helix] (1A2-V10 packaged phagemid).
In a specific embodiment, the conditional origin of replication is definitely an origin of replication, the replication of which depends on the presence of a number of specified protein, peptid, RNA, nucleic acid, molecule or any mix thereof.
It need to be noted that as used herein, the singular forms “a”, “an” and “the” include things like plural referents Until the context clearly dictates in any other case.
In a selected embodiment, claimed donor bacterial cell stably comprises a nucleic acid encoding mentioned rep protein, particularly said primase-helicase, stated nucleic acid normally comprising or consisting with the sequence SEQ ID NO: nine.
This is totally distinctive for the bacterial ORI, because it would mean that It could be Lively naturally and constitutively.
15. the tactic In accordance with embodiment 14, wherein stated conditional origin of replication is active in said donor bacterial cell due to the fact explained donor bacterial mobile expresses mentioned presented protein, peptid, nucleic acid, RNA, molecule or any combination thereof.
By “host organism” is meant herein any multicellular organism, such as any animal or any plant. In a selected embodiment, reported host organism is usually a host subject matter.
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stated molecule of curiosity may additional be made by claimed targeted receiver bacterial mobile in almost any sort. especially, claimed HMM might be selected with the group consisting of secreted molecules, intracellular molecules and membrane-displayed molecules.
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The current invention also concerns a way for 立即註冊並參與 ex vivo modulating a microbiome from an setting by collecting focused receiver bacterial cell from claimed atmosphere and by delivering a nucleic acid of desire into mentioned targeted receiver bacterial mobile of explained microbiome, stated nucleic acid of fascination generating a presented influence, as disclosed above, on mentioned qualified receiver bacterial mobile, whereby explained method comprises speaking to a nucleic acid vector comprising said nucleic acid of desire with explained microbiome, wherein said vector even more comprises a conditional origin of replication and that is inactive during the targeted receiver bacterial cell but is Energetic in a donor bacterial mobile, and claimed vector is devoid of antibiotic resistance marker,
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